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Figure 4 | BMC Biochemistry

Figure 4

From: Development of a sensitive non-radioactive protein kinase assay and its application for detecting DYRK activity in Xenopus laevis oocytes

Figure 4

Immunocomplex kinase assay of xDYRK1B from Xenopus laevis oocytes. Xenopus oocytes were lysed and the lysate was subjected to immunoprecipitation with either DYRK1B antiserum or preimmune serum (αD1B, Pre). Aliquots from immunocomplex kinase reactions (100 μM ATP, 50 μM tau207-219, 30°C, total volume of 50 μL) were taken at different times and the amounts of phosphorylated tau207-219 were determined by the ELISA method with the help of a standard curve. Error bars represent the difference between the duplicate measurements.

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BMC Biochemistry

ISSN: 1471-2091

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