Figure 6
From: Activity of the acyl-CoA synthetase ACSL6 isoforms: role of the fatty acid Gate-domains
Effect of Triton X-100 on activity. Activity measurements were performed with 5 μM (14C)-C18:1-OH as described in legend of Figure 2, with isoform 2 (F-Gate) (filled circles) and its N-terminus truncated version, ΔN-(F-Gate) (open circles), in presence of 0.1, 0.5, 1, 2, 3, 4 and 5 mM Triton X-100. Average and the standard deviation of 3 different experiments performed with each concentration are shown. Inset: Isoform 2 was incubated 30 min on ice with either buffer or 15.9 mM (1%) Triton X-100, as indicated. The treated enzyme preparation was then diluted in the reactional mixture to lower the concentration of Triton X-100 to a non-inhibitory concentration of 130 μM. To the reference sample incubated with buffer, Triton X-100 was added to the reaction at a concentration of 130 μM. Average and the standard deviation of 3 different experiments for each condition is shown.