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Figure 3 | BMC Biochemistry

Figure 3

From: The acidic domains of the Toc159 chloroplast preprotein receptor family are intrinsically disordered protein domains

Figure 3

Purified recombinant A-domains of atToc159 and atToc132 were analysed using circular dichroism (CD) and fluorescence spectroscopy. (A) Far-UV CD spectra of 132AHis and 159AHis at 25°C and pH 8.0. Temperature-dependent and pH-dependent far-UV CD spectra of 132AHis (B and C) and 159AHis (D and E) are also shown. Summary of the deconvoluted data is shown in Table 1. Intrinsic fluorescence of 132AHis excited at 295 nm was measured at pH 3.2 and 7.5 (inset, panel C).

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BMC Biochemistry

ISSN: 1471-2091

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