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Table 6 Effect of various Sulfhydryl reagents on CPRHG

From: Purification and characterization of cysteine protease from germinating cotyledons of horse gram

Conc. of reagents

(mM)

Relative activity (%)

 

Glutathione

Cysteine

β-ME

Dithiothretol

0.5

100

106

107

112

1.0

121

114

120

116

2.0

115

110

115

131

5.0

119

105

98

127

10.0

172

103

92

106

Control

100

100

100

100

  1. The hydrolyzing activity is expressed as per cent of control (19.73 units/h/mg enzyme)