Figure 1From: Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeatsCo-immunoprecipitation of endogenous PP6R3 and PP6c. (A) Immunoprecipitates (IP) were prepared from HeLa cells with pre-immune sheep serum (lane 1), blank beads (lane 2) or anti-PP6R3 sheep antibody (lane 3). Precipitates were analyzed by immunoblotting for PP6R3 (upper panel) and PP6c (lower panel). (B) Co-immunoprecipitation of PP6R3 and PP6c from HepG2 cells with anti-PP6R3 antibody (lane 1). The PP6R3 and PP6c remaining unbound were detected by immunoblotting the supernatant (S, lane 2) for PP6R3 (upper panel) and PP6c (lower panel)Back to article page