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Table 2 Rate constants (K2) of MutY mutants.

From: Insights into the role of Val45 and Gln182 of Escherichia coli MutY in DNA substrate binding and specificity

DNA

WT

V45A

Q182L

V45A/Q182L

A/G

0.24 ± 0.03a

0.19 ± 0.03 (0.80)b

0.025 ± 0.016 (0.11)

0.032 ± 0.026 (0.14)

A/GO

2.4 ± 0.3

1.3 ± 0.1 (0.54)

0.16 ± 0.02 (0.07)

0.37 ± 0.05 (0.15)

  1. ak2 values (Min-1) are means ± standard deviation for more than three experiments. k2 values with A/G and A/GO are determined at 37°C and 20°C, respectively.
  2. b The numbers in parenthesis are the folds of rate relative to wild-type (WT) MutY.
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BMC Biochemistry

ISSN: 1471-2091

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