TIPT2 and geminin interact with basal transcription factors to synergize in transcriptional regulation
© Pitulescu et al; licensee BioMed Central Ltd. 2009
Received: 06 January 2009
Accepted: 10 June 2009
Published: 10 June 2009
The re-replication inhibitor Geminin binds to several transcription factors including homeodomain proteins, and to members of the polycomb and the SWI/SNF complexes.
Here we describe the TATA-binding protein-like factor-interacting protein (TIPT) isoform 2, as a strong binding partner of Geminin. TIPT2 is widely expressed in mouse embryonic and adult tissues, residing both in cyto- and nucleoplasma, and enriched in the nucleolus. Like Geminin, also TIPT2 interacts with several polycomb factors, with the general transcription factor TBP (TATA box binding protein), and with the related protein TBPL1 (TRF2). TIPT2 synergizes with geminin and TBP in the activation of TATA box-containing promoters, and with TBPL1 and geminin in the activation of the TATA-less NF1 promoter. Geminin and TIPT2 were detected in the chromatin near TBP/TBPL1 binding sites.
Together, our study introduces a novel transcriptional regulator and its function in cooperation with chromatin associated factors and the basal transcription machinery.
The core promoter is defined as the genomic region required for recruitment of the transcription apparatus and can be considered as the priming stage for transcription initiation . Recruitment of RNA polymerase II requires the assembly of a preinitiation complex, including the basal transcription factors TFIIA, B, D, E, F and H near the transcriptional start site(s) . In addition, transcriptional regulation often involves TAFs (TATA-associated factors), mediator complex(es), and positive or negative cofactors, which associate with cis-acting DNA sequences often located further upstream or sometimes downstream of the start site . Only 10–20% of the promoters in mammals, Drosophila and Arabidopsis contain a TATA box, a conserved AT-rich sequence motif approximately 30 bp upstream from the first transcribed nucleotide [3–8]. In mammals, this minority of promoters is often associated with tissue-specific genes and high conservation across species [9, 10]. The TATA box associates with TBP, the TATA box binding protein, which as an element of the TFIID factor also binds to many TATA-less promoters [11–14]. Genomic screening approaches in yeast suggest that the presence of TBP in the chromatin near a promoter does not necessarily correspond to transcriptional activity [15–17]. The adenovirus major late promoter (AdMLP) is a classical example for a TATA box promoter. It contains immediately upstream and downstream of the box additional protein binding sequences, the G-rich TFIIB recognition elements (BREs), which were also found in TATA-less promoters [18–21].
A TBP-like factor, TBPL1 (TLP/TLF/TRF2) has no affinity to the TATA box, but binds to other promoter elements, probably with a TBP-like function . It was found in association with several promoters, e.g. of the PCNA, the wee1, the neurofibromytosis type 1 (NF1), the histone H1, and several ribosomal protein genes [23–30]. However, no common binding motif could so far be delineated in mammals or Drosophila.
Geminin is a cell cycle regulatory protein, which functions through an interaction with the replication licensing factor Cdt1 [31–33]. In addition, it was identified as a binding partner of developmental control proteins. It represses Hox functions during embryogenesis by direct association with Polycomb members on Hox regulatory chromatin elements, and by impairing Hox protein action on target genes through direct binding to the homeodomain [34, 35]. In a similar way, geminin directly interacts and antagonizes the role of another homeobox protein, Six3 . It represses the activator function of pro-neural basic helix-loop-helix transcription factors neurogenin 2 and NeuroD on their target neuron-specific genes by sequestering Brg1, the catalytic subunit of the chromatin remodeling complex SWI/SNF . Alternatively, geminin represses neuronal gene transcription in non-neural cells through its association with the AP4 transcription factor, by recruiting the co-repressor SMRT and histone deacetylase HDAC3 to neuronal gene promoters .
Here, we describe the function of TIPT2, a protein we identified through its interaction with geminin. Both geminin and TIPT2 bind to the basic transcriptional machinery, and can activate transcription synergistically. They were found, alone or together, associated with the chromatin of specific promoters.
We previously identified the polycomb factor Scmh1 and the homeodomain proteins Hoxd10 and Hoxa11 as geminin binding partners in a yeast two-hybrid screen of a day 8.5 mouse embryo cDNA library . In the same screen, a strongly interacting protein was identified, encoded by a 715 bp, poly(A)-tailed cDNA, corresponding to the Riken cDNA clone 5133400G04 [GenBank:NM_029485]. A database entry identified the protein as isoform 2 of "TIPT" (TATA-binding protein-like factor-interacting protein), more recently named TRF2 interacting protein in testis [GenBank:AAV97890] . TIPT is encoded by three differentially spliced variants, TIPT isoform 1 [GenBank:AAV97890] (185 amino acids), TIPT isoform 2 [GenBank:AAH60950] (205 amino acids) and TIPT isoform 3 [GenBank:BAB30197](159 amino acids). Mammalian TIPTs are strongly conserved, often represented by several isoforms, but related proteins were not found in other vertebrates or Drosophila. There are no similarities to other known proteins, and no conserved domain was obvious. Two coiled-coil regions (amino acids 50–106 and 128–168), known to be protein-protein interaction sites, were predicted by the Simple Modular Architecture Research Tool program http://smart.embl-heidelberg.de/.
In mouse embryonic stem cells, SNL and NIH 3T3 mouse fibroblasts, monkey Cos-7, RD rat rabdomyosarcoma, HeLa human cervical cancer, and U2OS human osteosarcoma cells TIPT2 was localized both in cytoplasm and nucleus, with particular enrichment in nucleoli (Figure 1F, Additional file 1, data not shown). There, it co-localized with nucleolar markers, such as the basal transcription factor TBPL1, nucleophosmin (NPM), and the 15.5 K protein (Snu13p in yeast) [40–42]. HA-tagged TIPT2 co-localized in nucleoli and nucleoplasma with GFP-TBP after co-transfection of expression vectors (Additional file 1).
TIPT2 interacting proteins
TIPT2 and TBP bind to separate DNA elements
TBP retarded significantly AdMLP oligonucleotides, which were clearly separated from TIPT2/AdMLP bands (Figure 3G, lanes 2,3). The TBP/AdMLP band completely disappeared when both TBP and TIPT2 were present in the binding reaction, while the TIPT2/AdMLP complex was maintained (Figure 3G, lane 4). More retarded oligonucleotides were observed above the TIPT2/AdMLP DNA complex, which may indicate the formation of a ternary TIPT2/TBP/DNA complex, but could not be defined in a clear band. When anti-TBP antibodies were added to this binding reaction, the TIPT2/DNA complex was still present, and even more retarded oligonucleotides were observed (Figure 3G, lane 5), possibly indicating that TIPT2 and TBP bind independently of each other to the AdMLP.
TIPT2, TBP and geminin synergize to activate the TATA box-containing promoters
Similar transfection experiments were performed comparing the non-mutated AdML promoter with mutants in the binding sites for either TBP (m1) or TIPT2 (m3). The background level achieved with single or double transfections was slightly elevated when TIPT2 and geminin vectors were transfected together (Figure 4C). Here, differences between the wild-type AdMLP reporter and reporters with a mutation in either the TIPT2-binding BRE element, or the TBP-binding TATA box were not recognizable. Double transfections did not produce a significant difference between the luciferase activities of BREu mutant or TATA box mutant and wild type AdML promoter (p-value > 0,01). Maximal luciferase activities were obtained in triple transfections using the wild-type form. Both the TATA box and BREu mutated promoters gave lower activation levels compared to the wild-type promoter (p-value < 0,001, respectively < 0,01; Figure 4C).
To investigate if TIPT2, TBP and geminin also activated a different TATA-containing promoter, the herpes virus thymidine kinase (TK) promoter was assayed, which does not contain a BRE element upstream of its TATA box. Still, the transfection results were quite similar to the AdMLP data. Single transfection of TBP, TIPT2 or geminin, as well as double transfections of TBP/TIPT2 or TBP/geminin activated expression only marginally (2–4 fold; Figure 4D). Triple transfections of TBP, TIPT2, and geminin activated the reporter very strongly (29 fold) and synergistically. This effect was TBP-specific, because it could not be obtained by adding TBPL1 (p-value < 0.01; Figure 4E).
The indicated reporter assays were realized in the presence of endogenous TIPT2, geminin and TBP, all three proteins being present in the U2OS cell line. We tried to knock-down endogenous TIPT2 using various small interfering (si) RNAs and short hairpin constructs. While TIPT2 RNA levels could be decreased more than 80%, the protein levels were not significantly affected even after several days and repeated siRNA transfection (data not shown). However, endogenous geminin levels could be knocked-down efficiently with specific siRNA molecules (Figure 4G). The experiments indicated that both single (TBP; p-value < 0,001) and double overexpressions (TIPT2/TBP; p-value < 0,001) were reduced in the absence of geminin (Figure 4F). Together, these results suggest that TIPT2 synergizes with geminin and TBP in order to activate TATA-containing promoters, both in the presence or absence of a TIPT2 binding DNA sequence element. We see significant, logic changes in the activation of TATA promoters in response to different protein combinations. It is however apparent, that we did not necessarily observe a complete abrogation, if one factor was missing, mutated, or its binding site was removed.
Association of TIPT2 and TBPL1-TFIIA with the neurofibromin promoter
TIPT2, TBPL1 and geminin synergize to activate the TATA-less NF1 promoter
We next analyzed whether TIPT2 was able to contribute to the activation of the NF1 promoter by employing luciferase reporter constructs with the NF1 promoter, containing multiple TBPL1 binding sites . TBPL1, TIPT2 or geminin alone were not capable of activating the reporter significantly (Figure 5B). TIPT2 and geminin together produced already a significant increase (20 fold), and the additional inclusion of TBPL1 led to a very strong, synergistic activation (44 fold; Figure 5B). Replacing TBPL1 by TBP revealed that the triple combination TBP/TIPT2/geminin was not more active than just TIPT2/geminin (p-value < 0,001), indicating that NF1 activation was TBPL1-specific (Figure 5B). A knock-down of endogenous geminin decreased the activity of the NF1 reporter in the presence of exogenous TBPL1 or TIPT2/TBPL1 to levels significantly below the control background in the absence of any siRNA transfected, by a factor of about 4 (p-value < 0,01, respectively < 0,001) (Figure 5C). This result suggests that medium levels of activation in the absence of transfected geminin benefited from the presence of endogenous geminin. In summary, we show that TIPT2, TBPL1 and geminin activate the TATA-less NF1 promoter construct synergistically.
The presence of TIPT2 and/or geminin in specific chromatin
Recently, information on a second isoform of TIPT (isoform 1) was independently reported . These authors describe expression in male germ cells, interaction with TBPL1 (TRF2), DNA binding and association with chromatin, in line with our findings on isoform 2. However, they did not detect the widespread expression we saw on the RNA and protein level for isoform 2, although probe and antibodies would be expected to crossreact.
In our study, we describe the synergistic function of two interacting factors, TIPT2 and geminin, in the activation of transcription. TIPT2 and geminin have in common that they can interact with polycomb group members (PcGs), which are known for their function in the maintenance of gene repression [47–52]. Therefore, it came unexpected that PcGs were found together with the basal transcriptional machinery on repressed promoters [49, 53]. It lead to the hypothesis that PcG proteins maintain silencing by inhibiting general transcription factor-mediated activation of transcription by interfering with the formation of the preinitiation complex . Our findings suggest that TIPT2 and geminin could be involved in the transition from inactive to active transcription via association with basal transcription as well as Polycomb factors. We found both geminin and TIPT2 in the chromatin of transcribed genes. TIPT2 was significantly enriched in nucleoli, a subcellular localization shared by the bHLH transcription factor Hand1, the basal transcription factor TBPL1, by Drosophila testis-specific TAF homologs and, albeit as an exception, by some PcGs [40, 55–57]. For the nuclear geminin, on the other hand, a nucleolar localization was not specifically described. If we observe a cooperation of these proteins in Pol II-dependent transcription, their functional interactions must occur in the nucleoplasm. Nucleoli might represent a site to keep transcription factors unavailable for gene regulatory functions, a hypothesis which may also apply to TIPT2. However, we have preliminary evidence that the nucleolar localization of TIPT2 also reflects a role in the activation of ribosomal RNA by polymerase I. In addition, our unpublished data indicate that TIPT2 nucleolar localization is regulated by phosphorylation.
Sequences flanking the core TATA box can influence the assembly of complexes, affect the basal level of transcription and the response to activators [58–60]. Our results show that TIPT2 contacts the AdMLP on a BRE site immediately upstream of the TATA box, without a need for TFIIB or TBP. In band-shift assays TIPT2/AdMLP complexes run in two or more bands (Figure 3A, lanes 2,3; 3D, lane 2; 3F, lane2). The faster migrating band represents TIPT2 associated with the BREu site of the oligonucleotide. The slower band(s) could represent more than one TIPT2 protein per oligonucleotide, with only one monomer engaged in DNA interactions. The slower migrating complex becomes more prominent than the faster complex after addition of cold oligonucleotides with BREu mutations (m2, m3, m4; Figure 3F). This might be indicative of a second DNA binding site on the TIPT2 protein interacting with the competitor.
The binding of TIPT2 to the BRE site interfered significantly with the binding of TBP to the TATA box. Both proteins on one oligonucleotide providing both binding sites were not clearly demonstratable. But some material of lower electrophoretic mobility may indicate the formation of a triple complex, containing AdMLP, TIPT2 and TBP. This disappeared upon addition of anti-TBP antibodies. ChIP experiments indicated that TBP and TIPT2 could be associated in vivo with chromatin in the TATA box containing promoter region of the GAPDH gene. While this finding does not formally prove the simultaneous presence, it appears very unlikely that the active GAPDH promoter exists in two different configurations, being occupied with either TBP or TIPT2.
Our data suggest that transcriptional activation by TIPT2 is optimal in the presence of a BREu element (AdMLP), but occurs also with a mutated (AdMLPm3) BREu element or in its absence (TK promoter). DNA binding of TIPT2 appears not to be essential, and association just by protein-protein contact seems to be sufficient. A recent bioinformatic study revealed that 24.5% of core promoters from the EPD (Eukaryotic Promoter Database: http://www.epd.isb-sib.ch), and 25.5% from the DBTSS (Database of Transcriptional Start Sites; http://dbtss.hgc.jp/index.html), respectively contain a BREu . More precisely, 28,1% of TATA-less promoters and 11.8% of TATA-containing promoters present a BREu. It is therefore conceivable that TIPT2 plays a role in the activation of transcription from many promoters.
The TIPT2 binding protein, geminin, activated transcription in several reporter assays. The simultaneous transfection of geminin and TIPT2 expression vectors boosted the activation of TATA-containing promoters. A mutant of TIPT2 that could not interact with geminin was clearly less active, again underlining the importance of the geminin/TIPT2 interaction in transcriptional activation. Our results concerning AdML promoter mutations analysis extends the previous findings that disruption of BREu decreased activated transcription . Our data indicate that a similar mechanism for transcriptional activation could hold true in the case of the TATA-less NF1 promoter [61, 62]. Mammalian TBPL1 does not stimulate transcription in vitro from TATA box-containing E4, AdML and Hsp70 promoters [24, 44, 63, 64]. We showed here that TIPT2, TBPL1 and NF1 promoter form a complex in vitro. The synergistic transcriptional activation of the NF1 promoter by TBPL1, TIPT2 and geminin is similar to the synergistic activation we observed for the TATA box-containing promoters (AdML, TK) by TBP, TIPT2 and geminin. It may, however, be too simplistic to correlate exclusively the TATA box promoters with TBP, and the TATA-less promoters with TBPL1 .
Down-regulation of geminin levels in human U2OS cells decreased the activity obtained from activated TK and NF1 promoters, indicating that already the endogenous geminin plays a significant role in our transcriptional assays. Geminin was reported to be involved in different, transcription-related complexes as a negative regulator of transcription, either directly or indirectly. In this study we show geminin's function as transcriptional co-activator. Other studies have shown that another chromatin remodeler, the SWI/SNF complex, is able to act in a gene-dependent manner either as activator or repressor [65, 66]. Our results suggest that geminin may interact with distinct protein complexes that exhibit cell-type or gene-specific functions. These interactions may decide if geminin affects transcription as a transcriptional repressor or activator.
In agreement with in vitro and reporter assay data, the chromatin analysis revealed geminin and TIPT2 on TATA-containing and TATA-less, active gene promoters. On the active endogenous chromatin, TIPT2 and geminin were present near the TATA box of the GAPDH gene, but not of the c-fos or the HSP-70 gene on a distance of two-three nucleosomes. The active TATA-less NF1 promoter is associated with geminin and TIPT2 in the region where TBPL1 was shown to bind. Our own ChIP evidence does not allow to conclude for the presence of TBP in the chromatin of the NF1 promoter in U2OS cells. However, recently the presence of TBP was described in the chromatin of the NF1 promoter in HeLa cells .
A genome-wide analysis of TRF2 recognition sites in Drosophila indicates that TRF2, a protein closely related to vertebrate TBPL1, plays a more general role than previously thought, being required for the expression of more than 1,000 genes, some involved in regulation of chromatin organization and cell growth (Histone H1 linker and ribosomal protein genes) . This may suggest also that in mammals TBPL1 plays an important role regulating essential cell functions not only in testis, but also in other tissues. In this new perspective, our study might indicate new factors binding to both TBP and TBPL1, which might constitute co-regulatory proteins common for both core promoter corresponding complexes.
In conclusion, we suggest that the two interacting proteins TIPT2 and geminin function as synergistic transcriptional regulators in contact with both the basal transcriptional machinery and chromatin factors, including members of the Polycomb complex.
The primers used for this study are listed in Additional file 2. The full-length TIPT2 clone was obtained from the mouse 8.5 cDNA library used in the yeast-two-hybrid screen, and included start codon, stop codon and poly A tail .
To construct mouse GST-TIPT2, TIPT2 was amplified from pPC86-TIPT2, and cloned into pGEX-KT. For the HA-TIPT2 expression construct, the coding sequence was inserted into the pcDNA vector (Invitrogen) containing an intron and a HA epitope (provided by R. Lührmann). The pFlag-HA-Myc-TIPT2 (FHM-TIPT2) construct was generated by inserting a TIPT2 PCR product in frame with the triple tag into the previously described pFHM-IRESNeo vector . For in vitro transcription/translation, SP6/T7 vectors were used or generated with the complete coding sequences for TIPT2, geminin, Scmh1 , Mel18, Ring1B (provided by H. Koseki), Mph2, TBPL, TBP , and TFIIB . GFP-TBP and GFP-TBPL1 plasmids were described before, and provided by J.A. Chong . The pCMV3-geminin construct was previously described , mutant geminin expression vectors lacking the coiled-coil (Gmnn m2), or the Brg1 binding domain (Gmnn m3), respectively, were provided by K. Kroll . Reporter plasmids were based on pGL3-Basic (Promega), such as pGL3-TK, pGL3-AdMLP (position -48 to +11; Figure 3E), pGL3-AdMLPm1 (AdML promoter mutated for TATA box; Figure 3E), pGL3-AdMLPm3 (AdML promoter mutated for TIPT2 binding; Figure 3E), and pGL3-AdE4 (position -56 to +39; Figure 3E). To construct the pGL3-NF1 reporter, first the human NF1 promoter region (position -335 to +15)  was amplified from human genomic DNA, then cloned into pGL3-Basic, and then, fourfold-reiterated oligonucleotides (position -282 to -259) were ligated into pGL3-NF1. A plasmid encoding GST-TIPT2 with a mutation in the geminin binding site (GST-TIPT2m) was generated from GST-TIPT2 plasmid by site directed mutagenesis. GFP-15.5 K was provided by R. Lührmann.
Whole cell extract was prepared from HeLa cells using a modified RIPA buffer (50 mM Tris, pH 7.4, 1% NP-40, 0,25% Na-deoxycholate, 0.5 mM EDTA, 150 mM NaCl, 1 mM PMSF). U2OS whole cell extract was prepared using lysis buffer (20 mM Tris, pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM PMSF). Testis extract was prepared from liquid nitrogen frozen testes of 10 weeks old male mice using testis lysis buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 0.5% NP-40, 1 mM PMSF). Organ extracts were prepared from freshly dissected P2 mice using lysis buffer containing 20 mM Tris, pH 7.5, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1 mM DTT and protease inhibitor cocktail. TFIIA-TBPL1 complex was purified according to , human TBP according to , GST-geminin and His-geminin according to . A peptide array (Jerini, Berlin) of overlapping 20-mers derived from the TIPT2 sequence was incubated with His-geminin and analyzed as described previously .
Recombinant TIPT2 and anti-TIPT2 antibodies
Full-length TIPT isoform 2 and a TIPT2 version mutated in the geminin binding site were produced as N-terminal GST fusions by standard procedures. Highly pure protein preparations were obtained by affinity chromatography on Glutathione Sepharose. GST-free TIPT2 was generated by thrombin cleavage on the gel matrix, and thrombin removal by benzamidine chromatography. TIPT2 was further purified from a preparative 15% SDS-PAGE gel, and its identity was confirmed by mass spectrometry. Gel-purified TIPT2 protein was used to generate polyclonal rabbit antibodies (Bioscience, Göttingen). The crude anti-TIPT2 rabbit serum was purified by chromatography on matrix coupled GST-TIPT2.
GST pull-down assay
Either radioactive proteins or whole cell extracts were used as preys. Radioactive binding proteins were generated by transcription/translation using the TNT SP6/T7 Coupled Reticulocyte Lysate System (Promega). For the preparation of the pull-down bait, Glutathione Sepharose-4B beads (Amersham) were pre-washed in lysis buffer (50 mM Tris pH 7.5, 500 mM NaCl, 2 mM EDTA, 5 mM DTT, 10% glycerol, 1 mM PMSF, Complete-EDTA protease inhibitors). For coupling, GST-TIPT2, GST-TIPT2m, GST-geminin, or GST, respectively, were incubated with Glutathione Sepharose-4B beads in binding buffer (20 mM Tris pH 7.5, 100 mM NaCl, 1 mM EDTA, 0,1% NP-40, 1 mM PMSF, protease inhibitors). GST protein-loaded beads were washed twice, incubated with radioactively labelled protein or protein lysates (from U2OS cells or adult mouse testis) for 1 hour at 4°C in 500 μl of binding buffer, washed twice in binding buffer, and then twice in washing buffer containing 150 mM NaCl. Proteins were eluted in 40 μl SDS loading buffer at 95°C. 20 μl of the eluted protein were electrophoresed, in parallel to an input lane containing 20% of the original radioactive protein preparation, 4% of total U2OS whole cell extract or 10% of testis extract.
Immunoprecipitations were performed using the anti-HA affinity matrix (Roche). U2OS and stable, HA-TIPT2 producing cells (HA-TIPT8) were used to prepare the protein extracts and to immunoprecipitate HA-TIPT2 and endogenous TBP. The cells were washed with cold PBS, and lysed with lysis buffer (50 mM Tris-HCl, pH 7.4, 120 mM NaCl, 1 mM EDTA, 1 mM EGTA, and 1% NP-40, 10 mM β-glycerol-P, 5 mM K2HPO4) by passage through the needles and incubating for 1 hour at 4°C. The extracts were centrifuged, and the supernatants were incubated with identical amounts of anti-HA agarose beads for several hours at 4°C. After washing (50 mM Tris HCl, pH 7.4, 150 mM NaCl, 0.1% NP-40), the complexes were eluted by boiling with 2× sample buffer (125 mM Tris HCl, pH 6.8, 4%SDS, 20% (v/v) glycerol, 0,004% bromphenol blue). 2.5% of the inputs and 50% from the eluates were electrophoresed.
The following primary antibodies were used: rabbit anti-TIPT2, raised against E.coli- expressed gel-purified full-length TIPT2; rabbit anti-geminin (sc-13015, Santa Cruz); mouse anti-TBP (3G3; gift from I. Davidson); mouse anti-TBP (Mab-TBPCSH-100, Diagenode); mouse anti-TBPL1 (2A1, provided by I. Davidson); rabbit anti-HA (ab9110, Abcam); rat monoclonal anti-HA (clone 3F10, 1867431, Roche); goat anti-Lamin B (sc-6216, Santa Cruz); mouse anti-GST (71097–3, Novagen); rabbit anti-acetyl histone H3 (06–599, Upstate); rabbit IgG (12–370, Upstate); mouse IgG (12–371, Upstate); mouse anti-NPM (32–5200, Zymed).
The following secondary antibodies were used: HRP-conjugated goat anti-rat IgG (112-035-143, Jackson ImmunoResearch); HRP-conjugated rabbit anti-goat IgG (ab6741-1, Abcam); HRP-conjugated goat anti-rabbit IgG (211–1303, Rockland); HRP-conjugated goat anti-mouse IgG (115-035-003, Jackson ImmunoResearch); Alexa Fluor 594 and 488 goat anti-rabbit IgG (A11012, A11008, Molecular Probes); Alexa Fluor 594 and 488 goat anti-mouse IgG (A-11005, A-11001, Molecular Probes); Alexa Fluor 594 chicken anti-rat IgG (A21471, Molecular Probes).
Electrophoretic mobility shift assays
In vitro interactions of human TBP, human TBPL1 complexed with TFIIA, recombinant GST-TIPT2 and His-geminin proteins with various promoter sequences were tested. The following, double-stranded oligonucleotides (see Additional file 2 and Figure 3E for sequences) were investigated: AdMLP oligonucleotide (position -40 to -16) , AdMLPm1-m9, NF1 oligonucleotide (position -288 to -253) [26, 72], AdE4 oligonucleotide . Binding reactions were performed at a final concentration of 5.5 nM labeled probe, no corrections for cpm were made. Competition by non-specific oligonucleotides in Figure 3A was performed at 0.03 μM poly(dI-dC), 0.33 μM poly(dG-dC), 0.08 μM or 0.4 μM double-stranded cold AdMLP. 0.1 μl of mouse anti-TBP (3G3) or mouse anti-GST antibodies (Novagen) were applied in the last seven minutes of the binding reactions (Figure 3B, G). For the competition experiments between AdMLP and TIPT2 by mutated versions of AdMLP double-stranded, unlabeled oligonucleotides were added at a final concentration of 1.6 μM or 5 μM, respectively, in the presence of 6 ng GST-TIPT2 (Figure 3F). Radiolabeled probe was added 15 min after starting the reaction.
Different binding reactions and electrophoresis conditions were performed for TBP or TBPL1, respectively. TBP binding reactions were performed in a final volume of 15 μl by mixing 3 μl 5× buffer (60 mM HEPES/KOH, pH 7.6, 300 mM KCl, 15 mM dithiothreitol, 1 mM EDTA, 10% polyethylene glycol 8000 (w/v), 20 mM MgCl2, 40% glycerol, 0.1% NP-40, 66.65 μg/ml BSA) with poly(dG-dC) (0.08 μM final concentration), 6 ng TBP, and labeled oligonucleotides (5.5 nM final concentration) .
The TBPL1 binding reactions were performed in a final volume of 15 μl by mixing 5 μl 3× buffer (60 mM HEPES/KOH, pH 7.6, 180 mM KCl, 9 mM dithiothreitol, 60% glycerol, 60 μg/ml BSA) with 200 ng BSA, poly(dG-dC) (0.03 μM final concentration), and 20 ng TBPL1-TFIIA complex . For checking the binding of both TBP and TIPT2 to AdMLP oligonucleotides, the proteins were added simultaneously to the binding reactions. The reactions were incubated at 30°C for 45 minutes. For the electrophoresis 4% polyacrylamide mini-gels (59:1 mono:bisacrylamide ratio, 5% glycerol, 1 mM DTT) were polymerized for minimum 1 hour and prerun for 45 minutes at 200 V and 4°C. For TBP bandshifts the gel was electrophoresed in 0.5× Tris-borate-EDTA (TBE) buffer supplemented with 5% glycerol  and 0.02% NP-40 for 20 minutes at 200 V at 4°C. The TBPL1 gel shift was electrophoresed in 0.5× TBE buffer for 16 minutes at 400 V and 4°C.
Cell culture and ChIP assay
For the stable production of tagged TIPT2 version, U2OS cells were transfected with HA-TIPT2 or FHM-TIPT2 expression constructs containing a Neomycin gene cassette. Clones were selected with 500 μg/ml neomycin (G418), and analyzed for HA-TIPT2 or FHM-TIPT2 expression.
1.5 × 107 HA-TIPT2 U2OS cells were used for 6 ChIP reactions. Disuccinimidyl glutarate (20593, Pierce) was applied to the washed cells at a final concentration of 2 mM in PBS pH 8.0, supplemented with 1 mM MgCl2for 30 min at RT. The cells were washed, 1.42% formaldehyde in PBS was applied for 20 min, and the reaction was stopped with glycine. Subsequently, cells were lysed in 1% SDS buffer, and the chromatin shearing was performed using the Bioruptor XL sonicator (Diagenode) at 4°C to obtain 200–500 bp DNA fragment size. Antibodies were used for immunoprecipitation of precleared chromatin. The complexes were collected using either protein G or protein A sepharose beads (16–201, 16–157, Upstate), the beads were washed, and the complexes were eluted. The crosslinking was reversed for 6 hours at 65°C. After RNase and proteinase K treatment the DNA was purified using the QIAquick PCR purification kit (Qiagen), and PCR reactions were performed. The primers used in the PCR reactions are described in Additional file 2.
U2OS cells were transfected in 24-well plates with the Fugene6 (Roche). After 36 h cells were processed for analysis with the Dual-Luciferase Reporter Assay System (Promega). A Renilla reporter (pRL-CMV, pRL-SV40, Promega) as internal standard was not included, since it turned out to be variably affected by introduced transcription factors. Each transfection experiment was performed in four parallel wells containing equal numbers of cells. The mean values, standard deviations and p-values were calculated. The p-values were obtained using a paired t-test. In text p-value < 0.01 indicates significant differences, and highly significant differences are presented as p-value < 0,001. The total amount of DNA per well was kept constant at 1425 ng by the addition of CMV-GFP. The reporter-TBP/TBPL1 expression vector ratio was always 10:1. Transfection experiments were repeated three times.
U2OS cells were transfected in 24-well plates with siGENOME SMARTpool human geminin (M-003270-01, Thermo Fisher Scientific) to obtain a 100 nM final concentration, using Dharmafect1 transfection reagent (Thermo Fisher Scientific). The control cells were treated in a similar way, except for the siRNA that was replaced by the siRNA dilution buffer (Roche). After 6 hours the cells were transfected with reporter and expression plasmids. After 24 h the cells were processed for analysis with the Dual-Luciferase Reporter Assay System (Promega).
We thank N.S. Asli, P. Collombat, W. Fischle, R. Klement, M. Konrad, E. Makarov, S. Pilarski and Y. Uerlings for discussions. We are grateful to J.A. Chong, D.E. Clapham, I. Davidson, R. Lührmann and K. Kroll for providing reagents. We thank S. Mahsur and P. Rus for excellent technical assistance, H. Urlaub and U. Plessman and M. Raabe for mass spectrometry. The work was supported by DFG and the Max-Planck Society (to M.E.P, L.L and M.K.), by a start-up grant from the Conseil Régional d'Aquitaine and the European Regional Development Fund (to M. T.), and by grants from The Ligue National contre le Cancer (to M. T.).
- Sandelin A, Carninci P, Lenhard B, Ponjavic J, Hayashizaki Y, Hume DA: Mammalian RNA polymerase II core promoters: insights from genome-wide studies. Nat Rev Genet. 2007, 8: 424-436. 10.1038/nrg2026.View ArticleGoogle Scholar
- Thomas MC, Chiang CM: The general transcription machinery and general cofactors. Crit Rev Biochem Mol Biol. 2006, 41: 105-178. 10.1080/10409230600648736.View ArticleGoogle Scholar
- Cooper SJ, Trinklein ND, Anton ED, Nguyen L, Myers RM: Comprehensive analysis of transcriptional promoter structure and function in 1% of the human genome. Genome Res. 2006, 16: 1-10. 10.1101/gr.4222606.PubMed CentralView ArticleGoogle Scholar
- Gershenzon NI, Ioshikhes IP: Synergy of human Pol II core promoter elements revealed by statistical sequence analysis. Bioinformatics. 2005, 21: 1295-1300. 10.1093/bioinformatics/bti172.View ArticleGoogle Scholar
- Ohler U, Liao GC, Niemann H, Rubin GM: Computational analysis of core promoters in the Drosophila genome. Genome Biol. 2002, 3: RESEARCH0087-10.1186/gb-2002-3-12-research0087.PubMed CentralView ArticleGoogle Scholar
- Ohler U: Identification of core promoter modules in Drosophila and their application in accurate transcription start site prediction. Nucleic Acids Res. 2006, 34: 5943-5950. 10.1093/nar/gkl608.PubMed CentralView ArticleGoogle Scholar
- Molina C, Grotewold E: Genome wide analysis of Arabidopsis core promoters. BMC Genomics. 2005, 6: 25-10.1186/1471-2164-6-25.PubMed CentralView ArticleGoogle Scholar
- Breathnach R, Chambon P: Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981, 50: 349-383. 10.1146/annurev.bi.50.070181.002025.View ArticleGoogle Scholar
- Carninci P, Sandelin A, Lenhard B, Katayama S, Shimokawa K, Ponjavic J, Semple CA, Taylor MS, Engstrom PG, Frith MC: Genome-wide analysis of mammalian promoter architecture and evolution. Nat Genet. 2006, 38: 626-635. 10.1038/ng1789.View ArticleGoogle Scholar
- Schug J, Schuller WP, Kappen C, Salbaum JM, Bucan M, Stoeckert CJ: Promoter features related to tissue specificity as measured by Shannon entropy. Genome Biol. 2005, 6: R33-10.1186/gb-2005-6-4-r33.PubMed CentralView ArticleGoogle Scholar
- Parker CS, Topol J: A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA-binding activity. Cell. 1984, 36: 357-369. 10.1016/0092-8674(84)90229-0.View ArticleGoogle Scholar
- Sawadogo M, Roeder RG: Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region. Cell. 1985, 43: 165-175. 10.1016/0092-8674(85)90021-2.View ArticleGoogle Scholar
- Smale ST, Kadonaga JT: The RNA polymerase II core promoter. Annu Rev Biochem. 2003, 72: 449-479. 10.1146/annurev.biochem.72.121801.161520.View ArticleGoogle Scholar
- Maston GA, Evans SK, Green MR: Transcriptional Regulatory Elements in the Human Genome. Annu Rev Genomics Hum Genet. 2006, 7: 29-59. 10.1146/annurev.genom.7.080505.115623.View ArticleGoogle Scholar
- Kim J, Iyer VR: Global role of TATA box-binding protein recruitment to promoters in mediating gene expression profiles. Mol Cell Biol. 2004, 24: 8104-8112. 10.1128/MCB.24.18.8104-8112.2004.PubMed CentralView ArticleGoogle Scholar
- Basehoar AD, Zanton SJ, Pugh BF: Identification and distinct regulation of yeast TATA box-containing genes. Cell. 2004, 116: 699-709. 10.1016/S0092-8674(04)00205-3.View ArticleGoogle Scholar
- Muller F, Demeny MA, Tora L: New problems in RNA polymerase II transcription initiation: matching the diversity of core promoters with a variety of promoter recognition factors. J Biol Chem. 2007, 282: 14685-14689. 10.1074/jbc.R700012200.View ArticleGoogle Scholar
- Lagrange T, Kapanidis AN, Tang H, Reinberg D, Ebright RH: New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor IIB. Genes Dev. 1998, 12: 34-44. 10.1101/gad.12.1.34.PubMed CentralView ArticleGoogle Scholar
- Qureshi SA, Jackson SP: Sequence-specific DNA binding by the S. shibatae TFIIB homolog, TFB, and its effect on promoter strength. Mol Cell. 1998, 1: 389-400. 10.1016/S1097-2765(00)80039-8.View ArticleGoogle Scholar
- Tsai FT, Sigler PB: Structural basis of preinitiation complex assembly on human pol II promoters. Embo J. 2000, 19: 25-36. 10.1093/emboj/19.1.25.PubMed CentralView ArticleGoogle Scholar
- Deng W, Roberts SG: A core promoter element downstream of the TATA box that is recognized by TFIIB. Genes Dev. 2005, 19: 2418-2423. 10.1101/gad.342405.PubMed CentralView ArticleGoogle Scholar
- Dantonel JC, Wurtz JM, Poch O, Moras D, Tora L: The TBP-like factor: an alternative transcription factor in metazoa?. Trends Biochem Sci. 1999, 24: 335-339. 10.1016/S0968-0004(99)01436-X.View ArticleGoogle Scholar
- Hochheimer A, Zhou S, Zheng S, Holmes MC, Tjian R: TRF2 associates with DREF and directs promoter-selective gene expression in Drosophila. Nature. 2002, 420: 439-445. 10.1038/nature01167.View ArticleGoogle Scholar
- Ohbayashi T, Shimada M, Nakadai T, Wada T, Handa H, Tamura T: Vertebrate TBP-like protein (TLP/TRF2/TLF) stimulates TATA-less terminal deoxynucleotidyl transferase promoters in a transient reporter assay, and TFIIA-binding capacity of TLP is required for this function. Nucleic Acids Res. 2003, 31: 2127-2133. 10.1093/nar/gkg315.PubMed CentralView ArticleGoogle Scholar
- Shimada M, Nakadai T, Tamura TA: TATA-binding protein-like protein (TLP/TRF2/TLF) negatively regulates cell cycle progression and is required for the stress-mediated G(2) checkpoint. Mol Cell Biol. 2003, 23: 4107-4120. 10.1128/MCB.23.12.4107-4120.2003.PubMed CentralView ArticleGoogle Scholar
- Chong JA, Moran MM, Teichmann M, Kaczmarek JS, Roeder R, Clapham DE: TATA-binding protein (TBP)-like factor (TLF) is a functional regulator of transcription: reciprocal regulation of the neurofibromatosis type 1 and c-fos genes by TLF/TRF2 and TBP. Mol Cell Biol. 2005, 25: 2632-2643. 10.1128/MCB.25.7.2632-2643.2005.PubMed CentralView ArticleGoogle Scholar
- Park KA, Tanaka Y, Suenaga Y, Tamura TA: TATA-binding protein-related factor 2 is localized in the cytoplasm of mammalian cells and much of it migrates to the nucleus in response to genotoxic agents. Mol Cells. 2006, 22: 203-209.Google Scholar
- Tanaka Y, Nanba YA, Park KA, Mabuchi T, Suenaga Y, Shiraishi S, Shimada M, Nakadai T, Tamura TA: Transcriptional repression of the mouse wee1 gene by TBP-related factor 2. Biochem Biophys Res Commun. 2007, 352: 21-28. 10.1016/j.bbrc.2006.10.175.View ArticleGoogle Scholar
- Bush SD, Richard P, Manley JL: Variations in intracellular levels of TATA binding protein can affect specific genes by different mechanisms. Mol Cell Biol. 2008, 28: 83-92. 10.1128/MCB.00809-07.PubMed CentralView ArticleGoogle Scholar
- Isogai Y, Keles S, Prestel M, Hochheimer A, Tjian R: Transcription of histone gene cluster by differential core-promoter factors. Genes Dev. 2007, 15: 2936-49. 10.1101/gad.1608807.View ArticleGoogle Scholar
- McGarry TJ, Kirschner MW: Geminin, an inhibitor of DNA replication, is degraded during mitosis. Cell. 1998, 93: 1043-1053. 10.1016/S0092-8674(00)81209-X.View ArticleGoogle Scholar
- Wohlschlegel JA, Dwyer BT, Dhar SK, Cvetic C, Walter JC, Dutta A: Inhibition of eukaryotic DNA replication by geminin binding to Cdt1. Science. 2000, 290: 2309-2312. 10.1126/science.290.5500.2309.View ArticleGoogle Scholar
- Tada S, Li A, Maiorano D, Mechali M, Blow JJ: Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin. Nat Cell Biol. 2001, 3: 107-113. 10.1038/35055000.PubMed CentralView ArticleGoogle Scholar
- Luo L, Yang X, Takihara Y, Knoetgen H, Kessel M: The cell-cycle regulator geminin inhibits Hox function through direct and polycomb-mediated interactions. Nature. 2004, 427: 749-753. 10.1038/nature02305.View ArticleGoogle Scholar
- Luo L, Kessel M: Geminin coordinates cell cycle and developmental control. Cell Cycle. 2004, 3: 711-714.View ArticleGoogle Scholar
- Del Bene F, Tessmar-Raible K, Wittbrodt J: Direct interaction of geminin and Six3 in eye development. Nature. 2004, 427: 745-749. 10.1038/nature02292.View ArticleGoogle Scholar
- Seo S, Herr A, Lim JW, Richardson GA, Richardson H, Kroll KL: Geminin regulates neuronal differentiation by antagonizing Brg1 activity. Genes Dev. 2005, 19: 1723-1734. 10.1101/gad.1319105.PubMed CentralView ArticleGoogle Scholar
- Kim MY, Jeong BC, Lee JH, Kee HJ, Kook H, Kim NS, Kim YH, Kim JK, Ahn KY, Kim KK: A repressor complex, AP4 transcription factor and geminin, negatively regulates expression of target genes in nonneuronal cells. Proc Natl Acad Sci USA. 2006, 103: 13074-13079. 10.1073/pnas.0601915103.PubMed CentralView ArticleGoogle Scholar
- Brancorsini S, Davidson I, Sassone-Corsi P: TIPT, a male germ cell-specific partner of TRF2, is chromatin-associated and interacts with HP1. Cell Cycle. 2008, 7: 1415-1422.View ArticleGoogle Scholar
- Kieffer-Kwon P, Martianov I, Davidson I: Cell-specific nucleolar localization of TBP-related factor 2. Mol Biol Cell. 2004, 15: 4356-4368. 10.1091/mbc.E04-02-0138.PubMed CentralView ArticleGoogle Scholar
- Smetana K, Ochs R, Lischwe MA, Gyorkey F, Freireich E, Chudomel V, Busch H: Immunofluorescence studies on proteins B23 and C23 in nucleoli of human lymphocytes. Exp Cell Res. 1984, 152: 195-203. 10.1016/0014-4827(84)90244-1.View ArticleGoogle Scholar
- Nottrott S, Hartmuth K, Fabrizio P, Urlaub H, Vidovic I, Ficner R, Luhrmann R: Functional interaction of a novel 15.5 kD [U4/U6.U5] tri-snRNP protein with the 5' stem-loop of U4 snRNA. Embo J. 1999, 18: 6119-6133. 10.1093/emboj/18.21.6119.PubMed CentralView ArticleGoogle Scholar
- Moore PA, Ozer J, Salunek M, Jan G, Zerby D, Campbell S, Lieberman PM: A human TATA binding protein-related protein with altered DNA binding specificity inhibits transcription from multiple promoters and activators. Mol Cell Biol. 1999, 19: 7610-7620.PubMed CentralView ArticleGoogle Scholar
- Teichmann M, Wang Z, Martinez E, Tjernberg A, Zhang D, Vollmer F, Chait BT, Roeder RG: Human TATA-binding protein-related factor-2 (hTRF2) stably associates with hTFIIA in HeLa cells. Proc Natl Acad Sci USA. 1999, 96: 13720-13725. 10.1073/pnas.96.24.13720.PubMed CentralView ArticleGoogle Scholar
- Nowak DE, Tian B, Brasier AR: Two-step cross-linking method for identification of NF-kappaB gene network by chromatin immunoprecipitation. Biotechniques. 2005, 39: 715-725. 10.2144/000112014.View ArticleGoogle Scholar
- Zeng PY, Vakoc CR, Chen ZC, Blobel GA, Berger SL: In vivo dual cross-linking for identification of indirect DNA-associated proteins by chromatin immunoprecipitation. Biotechniques. 2006, 41: 694-10.2144/000112297. 696, 698.View ArticleGoogle Scholar
- Pirrotta V: PcG complexes and chromatin silencing. Curr Opin Genet Dev. 1997, 7: 249-258. 10.1016/S0959-437X(97)80135-9.View ArticleGoogle Scholar
- Shao Z, Raible F, Mollaaghababa R, Guyon JR, Wu CT, Bender W, Kingston RE: Stabilization of chromatin structure by PRC1, a Polycomb complex. Cell. 1999, 98: 37-46. 10.1016/S0092-8674(00)80604-2.View ArticleGoogle Scholar
- Saurin AJ, Shao Z, Erdjument-Bromage H, Tempst P, Kingston RE: A Drosophila Polycomb group complex includes Zeste and dTAFII proteins. Nature. 2001, 412: 655-660. 10.1038/35088096.View ArticleGoogle Scholar
- Francis NJ, Kingston RE: Mechanisms of transcriptional memory. Nat Rev Mol Cell Biol. 2001, 2: 409-421. 10.1038/35073039.View ArticleGoogle Scholar
- Levine SS, Weiss A, Erdjument-Bromage H, Shao Z, Tempst P, Kingston RE: The core of the polycomb repressive complex is compositionally and functionally conserved in flies and humans. Mol Cell Biol. 2002, 22: 6070-6078. 10.1128/MCB.22.17.6070-6078.2002.PubMed CentralView ArticleGoogle Scholar
- Boyer LA, Plath K, Zeitlinger J, Brambrink T, Medeiros LA, Lee TI, Levine SS, Wernig M, Tajonar A, Ray MK: Polycomb complexes repress developmental regulators in murine embryonic stem cells. Nature. 2006, 441: 349-353. 10.1038/nature04733.View ArticleGoogle Scholar
- Breiling A, Turner BM, Bianchi ME, Orlando V: General transcription factors bind promoters repressed by Polycomb group proteins. Nature. 2001, 412: 651-655. 10.1038/35088090.View ArticleGoogle Scholar
- Dellino GI, Schwartz YB, Farkas G, McCabe D, Elgin SC, Pirrotta V: Polycomb silencing blocks transcription initiation. Mol Cell. 2004, 13: 887-893. 10.1016/S1097-2765(04)00128-5.View ArticleGoogle Scholar
- Chen X, Hiller M, Sancak Y, Fuller MT: Tissue-specific TAFs counteract Polycomb to turn on terminal differentiation. Science. 2005, 310: 869-872. 10.1126/science.1118101.View ArticleGoogle Scholar
- Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M: Nucleolar proteome dynamics. Nature. 2005, 433: 77-83. 10.1038/nature03207.View ArticleGoogle Scholar
- Martindill DM, Risebro CA, Smart N, Franco-Viseras Mdel M, Rosario CO, Swallow CJ, Dennis JW, Riley PR: Nucleolar release of Hand1 acts as a molecular switch to determine cell fate. Nat Cell Biol. 2007, 9: 1131-1141. 10.1038/ncb1633.View ArticleGoogle Scholar
- Wolner BS, Gralla JD: TATA-flanking sequences influence the rate and stability of TATA-binding protein and TFIIB binding. J Biol Chem. 2001, 276: 6260-6266. 10.1074/jbc.M008273200.View ArticleGoogle Scholar
- Wolner BS, Gralla JD: Roles for Non-TATA Core Promoter Sequences in Transcription and Factor Binding. Mol Cell Biol. 2000, 20: 3608-3615. 10.1128/MCB.20.10.3608-3615.2000.PubMed CentralView ArticleGoogle Scholar
- Chen Z, Manley JL: Core promoter elements and TAFs contribute to the diversity of transcriptional activation in vertebrates. Mol Cell Biol. 2003, 23: 7350-7362. 10.1128/MCB.23.20.7350-7362.2003.PubMed CentralView ArticleGoogle Scholar
- Viskochil D: Gene structure and expression. Neurofibromatosis type 1: From genotype to phenotype. Edited by: Upadhyaya M, Cooper DN. 1998, Oxford: BIOS Scientific, 39-63.Google Scholar
- Lee TK, Friedman JM: Analysis of NF1 transcriptional regulatory elements. Am J Med Genet A. 2005, 137: 130-135.View ArticleGoogle Scholar
- Ohbayashi T, Makino Y, Tamura TA: Identification of a mouse TBP-like protein (TLP) distantly related to the drosophila TBP-related factor. Nucleic Acids Res. 1999, 27: 750-755. 10.1093/nar/27.3.750.PubMed CentralView ArticleGoogle Scholar
- Ohbayashi T, Kishimoto T, Makino Y, Shimada M, Nakadai T, Aoki T, Kawata T, Niwa S, Tamura T: Isolation of cDNA, chromosome mapping, and expression of the human TBP-like protein. Biochem Biophys Res Commun. 1999, 255: 137-142. 10.1006/bbrc.1999.0159.View ArticleGoogle Scholar
- Murphy DJ, Hardy S, Engel DA: Human SWI-SNF component BRG1 represses transcription of the c-fos gene. Mol Cell Biol. 1999, 19: 2724-2733.PubMed CentralView ArticleGoogle Scholar
- Trouche D, Le Chalony C, Muchardt C, Yaniv M, Kouzarides T: RB and hbrm cooperate to repress the activation functions of E2F1. Proc Natl Acad Sci USA. 1997, 94: 11268-11273. 10.1073/pnas.94.21.11268.PubMed CentralView ArticleGoogle Scholar
- Dumay-Odelot H, Marck C, Durrieu-Gaillard S, Lefebvre O, Jourdain S, Prochazkova M, Pflieger A, Teichmann M: Identification, molecular cloning, and characterization of the sixth subunit of human transcription factor TFIIIC. J Biol Chem. 2007, 282: 17179-17189. 10.1074/jbc.M611542200.View ArticleGoogle Scholar
- Takada R, Nakatani Y, Hoffmann A, Kokubo T, Hasegawa S, Roeder RG, Horikoshi M: Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau). Proc Natl Acad Sci USA. 1992, 89: 11809-11813. 10.1073/pnas.89.24.11809.PubMed CentralView ArticleGoogle Scholar
- Malik S, Hisatake K, Sumimoto H, Horikoshi M, Roeder RG: Sequence of general transcription factor TFIIB and relationships to other initiation factors. Proc Natl Acad Sci USA. 1991, 88: 9553-9557. 10.1073/pnas.88.21.9553.PubMed CentralView ArticleGoogle Scholar
- Hoffmann A, Roeder RG: Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies. Nucleic Acids Res. 1991, 19: 6337-6338. 10.1093/nar/19.22.6337.PubMed CentralView ArticleGoogle Scholar
- Yu YT, Manley JL: Generation and functional analyses for base-substitution mutants of the adenovirus 2 major late promoter. Nucleic Acids Res. 1984, 12: 9309-9321. 10.1093/nar/12.24.9309.PubMed CentralView ArticleGoogle Scholar
- Hajra A, Martin-Gallardo A, Tarle SA, Freedman M, Wilson-Gunn S, Bernards A, Collins FS: DNA sequences in the promoter region of the NF1 gene are highly conserved between human and mouse. Genomics. 1994, 21: 649-652. 10.1006/geno.1994.1328.View ArticleGoogle Scholar
- Maldonado E, Ha I, Cortes P, Weis L, Reinberg D: Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex. Mol Cell Biol. 1990, 10: 6335-6347.PubMed CentralView ArticleGoogle Scholar
- Makino Y, Yogosawa S, Kayukawa K, Coin F, Egly JM, Wang Z, Roeder RG, Yamamoto K, Muramatsu M, Tamura T: TATA-Binding protein-interacting protein 120, TIP120, stimulates three classes of eukaryotic transcription via a unique mechanism. Mol Cell Biol. 1999, 19: 7951-7960.PubMed CentralView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.